We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 3G12 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects CD63. It targets an epitope within 23 amino acids from the C-terminal extracellular domain.

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal extracellular domain of human CD63.

Quality Control Testing

Evaluated by Western Blotting in SK-MEL-28 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CD63 in SK-MEL-28 cell lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CD63 in human bone marrow tissue sections.

Flow Cytometry Analysis: 1 µg from a representative lot detected CD63 in one million Human Peripheral Blood Mononuclear Cells (PBMCs)

Affinity Binding Assay: A representative lot of this antibody bound with CD63 peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

CD63 antigen (UniProt: P08962; also known as Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Lysosome integral membrane protein 1, Limp1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen Tetraspanin-30 (Tspan-30)) is encoded by the CD63 (also known as MLA1, TSPAN30) gene (Gene ID: 967) in human. CD63 is a member of the tetraspanin plasma membrane family, characterized by an N- and a C-terminal cytoplasmic domain (aa 2-11 and 225-238), four transmembrane domains (aa 12-32; 52-72; 82-102; 204-224) two extracellular loops (aa 33-51; 103-203) and an intracellular interconnection loop between transmembrane domains 2 and 3. Tetraspanins interact with each other and with many cell surface proteins to form a network of supramolecular complexes, also called tetraspanin-enriched microdomains (TEMs). They influence cellular processes, such as cell proliferation, migration, adhesion, differentiation, and motility. the activation of specific signaling pathways, depend on the associated proteins and cell types involved. Glycosylation plays a significant role in regulating the expression and function of these membrane proteins. CD63 expressed in most cells and tissue types and localized to the cytoplasmic membrane as well as in late endosomes, lysosomes, and multivesicular bodies. CD63 can be found in activated platelets, monocytes, granulocytes, dendritic cell and macrophages. Its expression can be upregulated in response to inflammatory stimuli and during certain immune responses. CD63 tetraspanin has been characterized as a binding partner of several integrins, but mainly of β1-integrin. The TIMP-1/CD63/β1-Integrin Complex is implicated in tumor progression and metastasis. Elevated levels of CD63 have been observed in conditions such as systemic lupus erythematosus (SLE) and other autoimmune diseases. CD63 is a prognostic marker in Glioblastoma multiforme, Liver hepatocellular carcinoma, Rectum adenocarcinoma. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Justo, B. L. and Jasiulionis, M. G., (2021), Int. J. Mol. Sci., 22(17), 9319; Dey, S., (2023), Adv Biol (Weinh), 7(7), 2300078)

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.