Anti-FLAG^® Antibody, clone 1I8 ZooMAb^® Rabbit Monoclonal

FLAG^®-tag, also known as a FLAG octapeptide or FLAG^® epitope, is an artificial synthetic peptide tag that is added to proteins using recombinant DNA technology. It is a versatile and widely used tool in molecular biology. The primary function of the FLAG^® tag is to facilitate the purification and detection of recombinant proteins. It is recognized by specific anti-FLAG^® antibodies, allowing for easy isolation and identification of FLAG^®-tagged fusion proteins through various techniques such as Western blotting, immunoprecipitation, ELISA, Flow cytometry and immunofluorescence. The FLAG^® -tag is made up of 8 amino acid sequence NH2-DYKDDDDK-COOH (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys). The sequence contains an enterokinase cleavage site. This sequence is derived from the N-terminus of the bacteriophage T7 gene 10 protein. Its small size and minimal impact on protein function make it an ideal choice for a variety of applications, including the study of disease-related proteins. This antibody reacts with N-terminal and C-terminal FLAG^® fusion proteins. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear extended peptide corresponding to FLAG® peptide sequence.

Quality Control Testing

Evaluated by Western Blotting with C-terminal FLAG-BAP fusion protein.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected FLAG^® in C-terminal FLAG-BAP fusion protein.

Tested Applications

Flow Cytometry Analysis: 1 µg from a representative lot detected FLAG^® tag in one million CHO-S cells transfected with FLAG ®-mTOR protein.

Affinity Binding Assay: A representative lot of this antibody bound FLAG^® peptide with at least one thousand-fold (1,000X) higher than with non-specific control peptide.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected FLAG^® tag in CHO-S cells transfected with FLAG^®-mTOR.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1I8 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects FLAG^® tagged proteins.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.