:
1109-125 (2019).
4. Belzer C. and de Vos WM., ISME J., 6:
1449–58 (2012).
5. Png CW., et al., Am J Gastroenterol., 105:
2420-28 (2010).
6. Zhang H., et al., Proc Natl Acad
23 °C No
8 60 minutes 100% Alcohol 23 °C No
9 60 minutes Xylene 23 °C No
10 60 minutes Xylene 23 °C No
11 30 minutes Paraffin 58 °C Yes
12 60 minutes Paraffin 58 °C Yes
13 90 minutes Paraffin 58 °C Yes
at 37 °C in a 96 well plate. After preincubation,
cells (normal human dermal fibroblasts at 0.5 x 105/mL)
were added to the antigen-antibody mixture (total
volume of 100 µL). The assay mixture was incubated
Q., et al., Thin-layer ion-exchange
chromatography of proteins. J. Chromatogr. A,
816(1), 97-105 (1998).
4. Kanyo, Z. F., et al., Crystallization and oligomeric
structure of rat liver arginase
Q., et al., Thin-layer ion-exchange
chromatography of proteins. J. Chromatogr. A,
816(1), 97-105 (1998).
4. Kanyo, Z. F., et al., Crystallization and oligomeric
structure of rat liver arginase
trichlorocarbanilide in control
of the protonmotive force in Escherichia coli cells.
Biochem. J., 212, 105-112 (1983).
5. Davidson, G.A., and Berma, M.C., Interaction of
valinomycin and monovalent cations
Stains, Dyes &
Indicators, Green, F. J., ed., Aldrich Chemical Co.
(Milwaukee, WI: 1990), p. 105.
MWM/JRC 8/03
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants
Schweizer, A., et al., J. Cell Biol., 107, 1643-1653
(1988).
2. Saraste, J., et al., J. Cell Biol., 105, 2021-2029
(1987).
3. Lahtinen, U., et al., J. Biol. Chem., 271, 4031-
4037 (1996).
Schweizer, A., et al., J. Cell Biol., 107, 1643-
1653 (1988).
2. Saraste, J., et al., J. Cell Biol., 105, 2021-2029
(1987).
3. Lahtinen, U., et al., J. Biol. Chem., 271, 4031-
4037 (1996).
4. Schweizer
of sin-
gle copy genes:
1 �g human genomic DNA = 3 × 105 target molecules
10 ng yeast DNA = 3 × 105 target molecules
1 ng E. coli DNA = 3 × 105 target molecules
Component Volume Concentration
optimal working dilution by titration test.
References
1. Rogers, J.H. et al., J Cell Biol., 105, 1845 (1987).
2. Parmentier, M., and Lafort, A., Eur. J. Biochem.,
196, 79 (1991).
3. Heizmann
be put in the same tube. For example, for n time points, put n x
2.5 x 105 cells(n x 625 µl at the cell density given in step 8) into one tube
for each treatment.
10.
Non-proliferating
cells
(e.g. hepatocytes)
1 x 106 cells/well 3 x 105 cells/well 5 x 10
4 cells/
well
Proliferating cells 2 x 105 cells/well 5 x 104 cells/well 5 x 10
3 cells/
well
Seeding
Progenitors, human 1 x >5 x 105 Cells Kit #HBeP.05
Corneal epithelium Progenitors, human 1 x >5 x 105 Cells Kit #HCeP.05
epidermal Keratinocyte Progenitors, pooled, human 1 x >5 x 105 Cells Kit #HPeKp.05
Baker, J.E., et al., Mol. Cell. Biol., 21, 2393-2403 (2001).
61. Horiba, M., et al., J. Clin. Invest., 105, 489-497 (2000).
62. Qi, M., et al., J. Biol. Chem., 276, 15868-15875 (2001).
63. Maeda, N., et al.
Regulation
of RAC GTPases". University of North Carolina at
Chapel Hill, Ph.D. dissertation, p. 105 (2019).
21. Tripler, Therese N., "Conservation and Divergence
Between P22-like Bacteriophages
of chromosome ends in
Saccharomyces cerevisiae". University of
Edinburgh, Ph.D. dissertation, p. 105 (2019).
20. Muraglia, Katrina Anne, "Amphotericin B ion
channels restore cystic fibrosis airway