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关键词:'111-50-2'
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分子克隆的问题解决
分子克隆是将目的基因 (GOI) 插入到质粒载体中的过程，然后将该载体插入到表达 GOI 编码的蛋白质的细胞中。一旦蛋白质在细胞中得到表达，这种表达即可用于各种不同的研究（如细胞信号传导、形态学或其他方面）。
葡聚糖
葡聚糖是葡萄糖酐聚合物。它由大约 95% 的alpha-D-(1-6)键合组成。其余的 a (1-3) 键合构成了葡聚糖的分支。分支长度的冲突数据意味着平均分支长度小于三个葡萄糖单位。但是，其他方法表明存在大于 50 个葡萄糖单位的分支。
半胱天冬酶抑制剂和活化剂
据报道可激活细胞半胱天冬酶的试剂包括化疗药物、TNF 受体激动剂和其他酶。细胞凋亡抑制剂是第一个确定的内源性半胱天冬酶抑制剂。
磷脂酶A2
磷脂酶A2（PLA2）代表的是一类可水解甘油磷脂的sn-2酯以产生脂肪酸和溶血磷脂的酶。目前已知的是，其中一些酶可在哺乳动物细胞中释放花生四烯酸以用于类花生酸的生物合成，因此PLA2抑制剂的开发引起了相当大的兴趣。基于氨基酸序列，现有超过12种不同的哺乳动物PLA2以及多种非哺乳动物的形式，它们被归类为具有多个亚组的14个组别。
Eppendorf
Eppendorf®品牌的锥形管和微量离心管、移液管和移液吸头、分液器、离心机、微量滴定板、搅拌器、光谱仪和实验室PCR设备。
胰蛋白酶抑制剂
天然胰蛋白酶抑制剂也称为丝氨酸蛋白酶抑制剂（serpins），是最大和最多样化的蛋白酶抑制剂家族。Serpins通过抑制体内丝氨酸蛋白酶来控制蛋白质的活化和分解代谢。
用于梯度离心的Ficoll® 400
Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。
基材和预制电子器件_电子材料_材料科学-默克生命科学
默克sigma-aldrich®的多种电化学部件选择包括各种具有易于处理的形状的水性/非水性参比电极，以及工作、对电极和辅助电极。此外，我们还为客户提供低成本、高精度设备，以方便您进行电池、太阳能电池氧化还原材料、超级电容器、电化学传感器和电化学能量收集器实验设计和测试。
使用PEPscreen®多肽研究AAV-hF.IX基因治疗的CTL MHC I类和Th II类免疫应答
重组腺相关病毒(AAV)载体介导的人凝血因子IX(hF.IX)表达基因治疗是一种前景可期的基因转移策略，因其使用肝脏特异性启动子，可实现hF.IX在肝脏中的长期表达和弱免疫应答。但在人类和某些动物中，F.IX表达会随着时间推移而下降，并且载体和转基因会诱发免疫应答。
胃肠道癌症中的免疫组织化学应用
结直肠癌是男性和女性中第三大常见癌症。预计2016年将发生136,000例结直肠癌。
PCR/qPCR/dPCR实验设计
整个PCR工作流程易受引入变量的影响。许多变量无法避免，例如样本来源和逆转录步骤的要求。实验设计也波动较大，可能决定PCR的成败，影响实验的可重复性和灵敏度。
杂化钙钛矿太阳能电池最新进展
近几十年来，人们对于环境可持续、商业可行的能源的迫切需求，催生并推动了大量致力实现低生产成本、高能效发电系统的研究工作。
MSI检测和IHC
结直肠癌是大多数西方国家居民中普遍发生的疾病，仅次于肺癌。
推荐单核细胞标准分离方法
以下操作步骤已接受Ficoll-Paque PLUS评估，推荐用于分离正常血样以尽可能提高可重现性。
RESOMER® Biodegradable Polymers for Medical Device Applications Research
Find use of RESOMER® biodegradable polymers in medical device applications research.
Syntheses of Functionalized Alkenes, Arenes, and Cycloalkenes via a Hydroboration-Coupling Sequence
Tandem hydroboration Suzuki Coupling both intermolecular and intramolecular gave diverse alkyl substituted products dppf
Trimethyl(trifluoromethyl)silane (Ruppert–Prakash Reagent)
Technical article for Trimethyl(trifluoromethyl)silane (Ruppert–Prakash Reagent)
Periodic Table of the Elements
Download the Periodic Table of the Elements with names, atomic mass and number in printable .pdf and image format. Use the periodic table chart by element names in alphabetical order for quick research, reference and lab use.
Glycol Ethers Properties & Examples, Organic Solvents
Glycol ethers, with both an ether and alcohol functional group in the same molecule, are one of the most versatile classes of organic solvents.
Pressure-Temperature Calculator for Solvents
Use the calculator to determine the boiling point or pressure of solvents based on the Antoine Equation and see solvent attributes. View a list of products based on the solvent CAS number.
PGM and PGM-free Catalysts for Polymer Electrolyte Membrane Fuel Cells
In this review, we highlight the recent progress in both PGM and PGM-free ORR catalysts for PEMFC applications. In addition, the remaining challenges in ORR catalyst development that meet the targets of PEMFC applications beyond the preliminary half-cell test are
Single-Walled Carbon Nanohorn Properties & Applications
Single-walled carbon nanohorns(SWCNHs) - Find manufacturing, material properties, and applications of carbon nanohorns.
Organic Dyes for Efficient and Stable Dye-Sensitized Solar Cells
Over the last decade, dye-sensitized solar cells (DSSCs) have attracted much attention because these unconventional solar cells exhibit high performance and have the potential for low-cost production.
Simplified LC-MS/MS Method for Glyphosate, AMPA, and Glufosinate in Oat-Based Cereals
A simplified LC-MS/MS method to determine glyphosate and related compounds using a Supel™ Carbon LC U/HPLC column for stability under higher pH conditions and sufficient retention for the polar analytes in the presence of methanol as an extraction solvent.
Analysis of Isocyanates Using the ASSET™ EZ4-NCO Dry Sampler
The unique sampler design ensures that both the vapor phase and particulate isocyanates are captured and derivatized during sampling.
Biomedical Implant Devices Fabricated from Low Young’s Modulus Titanium Alloys Demonstrating High Mechanical Biocompatibility
Biomedical implants are essentially foreign substances within the human body that must survive many years’ exposure to demanding mechanical and physiological conditions. Despite these challenges, metal implants have been widely used to substitute for or rebuild hard tissues such as
Poly(2-Oxazoline)s: The Versatile Polymer Platform for Biomedicine
The introduction of polymers into the biomedical field has opened new avenues in tissue engineering, implant design, biosensing, and drug delivery.
Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading
Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.
Analysis of Pesticide Residues in Ginger Powder Using QuEChERS Extraction and Clean-up with a Novel Dual Layer SPE Cartridge
The odor and pungency of ginger is due to the presence of terpenes, gingerols and shogaols. These compounds contribute to the highly complex matrix of ginger, which subsequently presents a challenge in low level analyses of contaminants. A novel 2-layer
Properties of Mononuclear Cells Isolated by the Ficoll-Paque Method
The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.
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