Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

Separation Options with Sephacryl®

Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.

Refolding of proteins using gel ﬁltration

This page shows how to refold proteins using gel filtration and products from Cytiva.

Genomic DNA Purification using illustra™ GenomicPrep Mini Spin Kits

This page shows Genomic DNA purification using illustra™ genomicPrep Mini Spin Kits from Cytiva.

Protocol Guide: Immunofluorescent Staining of Whole-Mount Organoids using Antibodies

How to stain organoids? A complete step-by-step protocol for immunofluorescent (IF) and immunocytochemical (ICC) staining of organoid cultures using antibodies

Performing a Purity and Homogeneity Check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Affinity Purification with Ultrafree^®-MC Centrifugal Filters

Affinity interaction chromatography is an effective method for protein purification that can achieve up to 95% purity in one step

Electron Microscopy

Electron microscopy utilizes a scanning or transmission system to pass an electron beam through a specimen to obtain ultrahigh resolution images with detailed structural information at the surface or atomic level.

Sepharose Big Beads: Purification from Crude, Viscous Samples at Large Scale

This page covers how and why to use Sepharose Big Beads for purification from crude, viscous samples.

Performing a Separation with Superdex

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Complete Workflow for Comprehensive Cannabis Terpenes Analysis

Complete workflow for the comprehensive analysis of terpenes in cannabis

BIOshell™ A400 Protein C4 U/HPLC Columns

BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.

Cancer Stem Cell Tumorsphere Formation Protocol

Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.

Rugged and Reproducible Solid Phase Microextraction (SPME) Sampling

SPME fibers with Carboxen®/Polydimethylsiloxane (CAR/PDMS) and Polydimethylsiloxane/ Divinylbenzene (PDMS/DVB) coatings are now available on a nitinol core (NIT) in a SS assembly for improved fiber performance.

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

Rapid Determination of Protein Binding Affinity Using Solid Phase Microextraction

Accurate protein binding affinities were measured using bioSPME more rapidly than membrane dialysis (RED) techniques. Binding affinities correlated to the reference values.

Virus Concentration by Ultrafiltration

Ultrafiltration using Amicon® Ultra centrifugal devices for concentrating virus in preparation of high titer virus stocks and isolation of virus from samples.

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