beta
signaling in normal and cancer cells. Mol. Cancer
Res., Mar 2(3), 183-95 (2004).
5. Abrahamson M., Meth. Enzymol., 244, 685-700
(1994).
6. Rivenbark, A.G. et al., Frontiers Biosci., 14, 453
the column for 2 minutes at 700 × g. The
purified sample is collected at the bottom of the
support tube (fraction 1).
11. Elute the column with PBS, pH 7.4. Place the
column into tube 2 and apply
compatible
with a range of standard receiver
96-well microtiter plates including
300 µL, 700 µL deep well and
150 µL conical well.
MultiScreen with Ultracel-10
membrane is ideal for applications
absorptivity of D(�)-Luciferin at 327 nm is 18.2 mmol-1
� L � cm-1).
For instance, to prepare a 700 �M solution of D(�)-Luciferin (8):
� Add 1.5 mg of D (�)-Luciferin to 5 ml of 70 mM Tris-acetate
Microbiological Criteria for Foods, Journal of Food Protection 57 (12), 1101-
1121 (1994)
5. M.B. Skirrow, British Medical Journal 2, 9-11 (1977)
6. D.E. Post, Food-Borne Pathogens Monograph Number
. Rybakin, V., and Clemens, C.S., Bioessays, 27,
625-632 (2005).
2. Ahmed, Z. et al., J. Histochem. Cytochem., 55, 687-
700 (2007).
3. Yan, M. et al., J. Immunol., 178, 5769-5778 (2007).
4. Nal
. Rybakin, V., and Clemens, C.S., Bioessays, 27,
625-632 (2005).
2. Ahmed, Z. et al., J. Histochem. Cytochem., 55, 687-
700 (2007).
3. Yan, M. et al., J. Immunol., 178, 5769-5778 (2007).
4. Nal
BCS 2 drugs.
Conclusions
The lubrication efficacy of gold-standard hydrophobic MST cannot be
completely matched with hydrophilic Poloxamer 188, however, good ejection
forces of less than 700 N can
and biologically
active component in basement membrane, is a complex
extracellular glycoprotein of 700-900 kDa that plays an
important role in many aspects of the cell biology.4, 5 It
is composed of one
2002) 36:57-68.
29) Henshall, D.C. et al., J. Neuroscience (2002) 22:8458-8465.
30) Chavez, J. and J. LaManna, J. Neuroscience (2002) 22:8922-8931.
31) Ji, R-R, et al., Neuron (2002) 36:57-68.
32