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DNA and RNA purification

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facet applications:DNA and RNA purification
facet content type:Protocol
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不含脱氧核糖核酸酶(DNase)的核糖核酸酶(RNase)方案
0.1 mU不含脱氧核糖核酸酶的核糖核酸酶,在温度为+37 °C、反应体积为50μL的PCR级水中,于30 min内可降解1 μg核糖核酸(RNA)。不含脱氧核糖核酸酶的核糖核酸酶蛋白含量为0.5 μg/μL。
TRI Reagent® 实验方案
TRI试剂是一种单步RNA分离试剂,可同时分离DNA、RNA和蛋白质。本实验方案概述了样品制备方法,以及各种样品提取方法和故障排除方案。
寡核苷酸的操作处理和稳定性
通过操作处理和稳定性技术来减少变量并确保无故障实验。
GenElute™细菌基因组DNA试剂盒实验方案(NA2100、NA2110、NA2120)
的GenElute™细菌基因组DNA试剂盒提供了一种从细菌中分离纯基因组DNA的简便方法。
GenElute™ 植物基因组DNA Miniprep试剂盒实验方案(G2N10, G2N70, G2N350)
本方案描述了使用GenElute植物基因组DNA Miniprep试剂盒,从多种植物中分离纯DNA的简单、便捷操作步骤。
REDExtract-N-Amp™ 植物PCR试剂盒实验方案
Description:REDExtract-N-Amp植物PCR试剂盒含有预混液形式的惰性上样染料,可在扩增后轻松凝胶上样。本实验方案提供了一种采用优化的预混液来快速提取扩增用植物基因组DNA的操作方法。
REDExtract-N-Amp™组织PCR试剂盒实验方案
exrract-N-Amp试剂盒实验方案提供了一种快速的DNA提取方法,可在15分钟内获得PCR样品。优化的PCR ReadyMix能成功产生清洁的扩增。RED上样染料实现样品跟踪。
RNAstable®实验方案
RNAstable是一种独特的储存介质,可在室温下保存RNA样品数月。使用RNAstable,您可以通过简单的重新水化,即只需加水便可完全恢复您的RNA样品。再水化的样品可立即使用,无需进一步纯化。
质粒DNA纯化
分子生物学研究使用从基因组DNA、蛋白质、核糖体和细菌细胞壁中纯化的质粒。质粒DNA纯化有多种方法。
GenElute™ 哺乳动物基因组 DNA 微量提取试剂盒实验方案
GenElute 哺乳动物基因组 DNA 纯化方案阐述了从多种哺乳动物来源中分离纯高分子量 DNA 的方法。
FFPE 组织
存档的福尔马林固定和石蜡包埋 (FFPE) 组织样本是分析基因表达和研究多种疾病的宝贵资源。
REDExtract-N-Amp™ Tissue PCR Kit Protocol
The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.
DNAgard® Blood Protocol
DNAgard Blood is designed for shipping and storage of DNA in mammalian whole blood. DNA in blood is protected by the unique stabilization properties of DNAgard Blood
RNase, DNase-free Protocol
0.1 mU RNase, DNase-free degrades 1 μg RNA in 30 min at + 37 °C in a reaction volume of 50 μL PCR grade water. The protein concentration of RNase, DNase-free is 0.5 μg/μL.
Plant RNA/DNA Purification Kit
The Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and DNA simultaneously from a single sample of plants.
Protein Sample Concentration
We provide gentle, membrane-based Protein Concentration options to concentrate proteins without compromising yield, even when labile or low in abundance.
Plant/Fungi DNA Isolation Kit Protocol
The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.
GenElute™ PCR Clean-Up Procedure
Follow this procedure to rapidly purify single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from excess primers, nucleotides, DNA polymerase, oil and salts using the GenElute™ PCR Clean-up Kit.
TRI Reagent® Protocol
TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.
Spermine Tetrahydrochloride for Molecular Biology
This product is suitable for DNA precipitation from low salt aqueous buffers. It has been tested for the absence of proteases and nucleases.
RNA Stabilization at Room Temperature in RNAstable®
Quantitative RT-PCR analysis demonstrates successful amplification of RNA templates that were stored dry in RNAstable® for 29 months at room temperature and under accelerated aging conditions equivalent to 12 years of room temperature storage.
Genomic DNA Purification from Sample Applied to FTA Cards
This page shows genomic DNA purification from sample applied to FTA cards from Cytiva.
Nucleic Acid Sample Preparation Methodology
Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation.
Genomic DNA Purification from Sample on FTA Elute
This page shows genomic DNA purification from sample on FTA elute from Cytiva.
Genomic DNA Purification using illustra™ GenomicPrep Mini Spin Kits
This page shows Genomic DNA purification using illustra™ genomicPrep Mini Spin Kits from Cytiva.
DNA Cleanup using illustra™ GFX Kits
DNA cleanup using illustra™ GFX kits from Cytiva.
Rapid Genotyping of Mouse Tissue Using Extract-N-Amp Tissue PCR Kit
Genomic detection of DNA via PCR amplification and detection on an electrophoretic gel is a standard way that the genotype of a tissue sample is determined. Conventional preparation of tissues for PCR-ready DNA often take several hours to days, depending
DNAstable® Protocol
DNAstable is a unique storage medium that preserves genomic DNA, plasmids, bacterial artificial chromosomes (BACs), PCR products and oligonucleotides at room temperature.
Oligonucleotide Handling & Stability
Handing and stability techniques to reduce variation and ensure trouble-free experiments.
GenElute™ RNA/DNA/Protein Plus Purification Kit
GenElute RNA/DNA/Protein Plus Purification Kit provides a rapid method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
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