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Restriction Enzyme Cloning Manual Buffer Recipes
TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer
Oligonucleotide Handling & Stability
Handing and stability techniques to reduce variation and ensure trouble-free experiments.
Yeast Transformation Protocols
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.
A highly efficient method to concentrate DNA for forensic STR genotyping using DNAstable®
Forensic laboratories routinely use STR genotyping for identity testing of biological samples. However, forensic samples often contain low copy numbers of target DNA, making it difficult to obtain complete STR profiles.
Genomic DNA Purification from Sample Applied to FTA Cards
This page shows genomic DNA purification from sample applied to FTA cards from Cytiva.
RNA Handling When Using the First Strand cDNA Synthesis Kit for RT-PCR (AMV)
First Strand cDNA Synthesis Kit for RT-PCR (AMV).
Preparing Cells for Electroporation
Preparing Cells for Electroporation
HighPrep™ PCR Clean Up Kit and Purification System Protocol
HighPrep™ PCR purification magnetic clean-up beads for manual and automated post-PCR, post-enzymatic reaction, NGS library clean-up.
Technical Bulletins
Technical bulletins for our shRNA products, including Bacterial Glycerol Stock, Purified Plasmid DNA Format, Lentiviral Format, MISSION® Control Vectors, MISSION Control Transduction Particles, MISSION Lentiviral Packaging Mix.
QuickLink™ DNA Ligation Kit
T4 DNA ligase is used for the joining of DNA molecules with compatible cohesive (sticky) termini, joining of blunt ended double stranded DNA molecules
DNA Detection
Nancy-520 for DNA Detection and Quantitation
DNAstable® Protocol
DNAstable is a unique storage medium that preserves genomic DNA, plasmids, bacterial artificial chromosomes (BACs), PCR products and oligonucleotides at room temperature.
General Considerations for Plasmid DNA Preparation
This page discusses methods for plasmid DNA purification and the factors impacting resulting DNA purity and yield using illustra plasmidPrep Kits from Cytiva.
Download The Free Biological Buffer Brochure
We offer an extensive portfolio of biological buffers backed by in-depth scientific knowledge, analytical capabilities, and technical support: strengthened by decades of manufacturing these critical products.
OverExpress™ Electrocompetent Cells
OverExpress™ Electrocompetent Cells are provided in 25 μL aliquots, sufficient for one transformation reaction.
Dehydrated and Ready-to-Use Media Request Information
Dehydrated and Ready-to-Use Media Request Information
Nucleic Acid Sample Preparation Methodology
Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation.
Scientific Poster: Addressing Handling Challenges of Chemicals by Dry Granulation
Authors: Thomas Briel, Raphael Guebeli, Rupa Bhattarai, Anke Simon, Corinna Merkel, Moritz Beck-Broichsitter
Inducible shRNA Vectors
Explore the benefits of IPTG-inducible vectors through performance data. These vectors offer regulated expression, which is important when studying essential and lethal genes.
Reliable DNA extraction from Whatman™ FTA cards
This study examined the yield and quality of DNA from samples applied to Whatman FTA cards, using five common methods of DNA extraction.
GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) - Protocol
GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA
Complete Whole Transcriptome Amplification Kit Protocol (WTA2)
WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias
Chemical Compatibility Testing
Our physical and chemical compatibility testing services help you comply with GMP guidelines worldwide, proving that your biopharmaceutical filters and single-use systems are not reactive, additive, or absorptive upon contact with drug product.