跳转至内容
Merck
CN
  • Uclacyanin Proteins Are Required for Lignified Nanodomain Formation within Casparian Strips.

Uclacyanin Proteins Are Required for Lignified Nanodomain Formation within Casparian Strips.

Current biology : CB (2020-08-29)
Guilhem Reyt, Zhenfei Chao, Paulina Flis, Isai Salas-González, Gabriel Castrillo, Dai-Yin Chao, David E Salt
摘要

Casparian strips (CSs) are cell wall modifications of vascular plants restricting extracellular free diffusion into and out of the vascular system [1]. This barrier plays a critical role in controlling the acquisition of nutrients and water necessary for normal plant development [2-5]. CSs are formed by the precise deposition of a band of lignin approximately 2 μm wide and 150 nm thick spanning the apoplastic space between adjacent endodermal cells [6, 7]. Here, we identified a copper-containing protein, Uclacyanin1 (UCC1), that is sub-compartmentalized within the CS. UCC1 forms a central CS nanodomain in comparison with other CS-located proteins that are found to be mainly accumulated at the periphery of the CS. We found that loss-of-function of two uclacyanins (UCC1 and UCC2) reduces lignification specifically in this central CS nanodomain, revealing a nano-compartmentalized machinery for lignin polymerization. This loss of lignification leads to increased endodermal permeability and, consequently, to a loss of mineral nutrient homeostasis.

材料
货号
品牌
产品描述

Sigma-Aldrich
二甲基亚砜, for molecular biology
Sigma-Aldrich
乙酸, glacial, ReagentPlus®, ≥99%
Sigma-Aldrich
MES 水合物, ≥99.5% (titration)
Sigma-Aldrich
尿素, powder, BioReagent, for molecular biology, suitable for cell culture
Sigma-Aldrich
IGEPAL® CA-630, for molecular biology
Sigma-Aldrich
乙二醇-双(2-氨基乙醚)-N,N,N′,N′-四乙酸, for molecular biology, ≥97.0%
Sigma-Aldrich
苯酚:氯仿:异戊醇 25:24:1,10 mM Tris(pH 8.0)、1 mM EDTA饱和, Supplied with Equilibration buffer, for molecular biology
Sigma-Aldrich
氢氧化钾, BioXtra, ≥85% KOH basis
Sigma-Aldrich
盐酸胍 溶液, 8 M, pH 8.5, buffered aqueous solution
Sigma-Aldrich
脱氧胆酸钠, ≥97% (titration)
Sigma-Aldrich
硫酸镁 七水合物, BioUltra, ≥99.5% (KT)
Sigma-Aldrich
多聚甲醛, reagent grade, crystalline
Sigma-Aldrich
木糖醇
Supelco
甲醇, analytical standard
Sigma-Aldrich
胡椒酸, 99%
Sigma-Aldrich
PIPES 二钠盐, ≥99% (titration)
Sigma-Aldrich
硝酸-14N 溶液, ~10 N in H2O, 99.99 atom % 14N