跳转至内容
Merck
CN

Biotinylated Micro-RNA Pull Down Assay for Identifying miRNA Targets.

Bio-protocol (2017-05-05)
Pornima Phatak, James M Donahue
摘要

microRNA (miRNA) directly associates with its target transcripts (mRNA). This protocol describes a method for detection of direct interaction between miRNA and mRNA. The result of interaction helps screening the specific target mRNAs for a miRNA.

材料
产品编号
品牌
产品描述

Sigma-Aldrich
乙醇,Pure 200纯度, Molecular Biology
Sigma-Aldrich
氯仿, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
Sigma-Aldrich
2-丙醇, BioReagent, ≥99.5%, Molecular Biology
Sigma-Aldrich
IGEPAL® CA-630, Molecular Biology