Oncogenic Transformation of Human-Derived Gastric Organoids.

Oncogenic Transformation of Human-Derived Gastric Organoids.

Methods in molecular biology (Clifton, N.J.) (2016-08-20)

Nina Bertaux-Skeirik, Jomaris Centeno, Jian Gao, Joel Gabre, Yana Zavros

摘要

The culture of organoids has represented a significant advancement in the gastrointestinal research field. Previous research studies have described the oncogenic transformation of human intestinal and mouse gastric organoids. Here we detail the protocol for the oncogenic transformation and orthotopic transplantation of human-derived gastric organoids.

材料

产品编号

品牌

产品描述

H0887

Sigma-Aldrich

羟乙基哌嗪乙硫磺酸溶液, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

A9165

Sigma-Aldrich

N-乙酰基-L-半胱氨酸, BioReagent, suitable for cell culture

Y0503

Sigma-Aldrich

Y-27632 二盐酸盐, ≥98% (HPLC)

C9891

Sigma-Aldrich

胶原酶来源于溶组织梭菌, Type IA, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid, For general use

H3884

Sigma-Aldrich

透明质酸酶来源于牛睾丸, Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid