- SCON-a Short Conditional intrON for conditional knockout with one-step zygote injection.
SCON-a Short Conditional intrON for conditional knockout with one-step zygote injection.
Experimental & molecular medicine (2022-12-10)
Szu-Hsien Sam Wu, Heetak Lee, Réka Szép-Bakonyi, Gabriele Colozza, Ayse Boese, Krista R Gert, Natalia Hallay, Ji-Hyun Lee, Jihoon Kim, Yi Zhu, Margot M Linssen, Sandra Pilat-Carotta, Peter Hohenstein, Hans-Christian Theussl, Andrea Pauli, Bon-Kyoung Koo
PMID36494589
摘要
The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.