跳转至内容

CN

Porcine synapsin 1: SYN1 gene analysis and functional characterization of the promoter.

Porcine synapsin 1: SYN1 gene analysis and functional characterization of the promoter.

FEBS open bio (2013-11-20)

Claus Hedegaard, Kasper Kjaer-Sorensen, Lone Bruhn Madsen, Carina Henriksen, Jamal Momeni, Christian Bendixen, Claus Oxvig, Knud Larsen

摘要

Synapsin 1 (SYN1) is a phosphoprotein involved in nerve signal transmission. The porcine SYN1 promoter orthologue was cloned and characterized to provide a means of expressing a transgene specifically in neurons. The nucleotide sequence of the promoter displayed a high degree of conservation of elements responsible for neuron-specific expression. Expression analysis of SYN1 demonstrated presence of transcript during embryonic development. Analysis of GFP expression in transgenic zebrafish embryos suggests that the pig SYN1 promoter directs expression in neuronal cells. Thus, the SYN1 promoter is a good candidate for use in the generation of pig models of human neurodegenerative disorders.

材料

Product Number

品牌

产品描述

Sigma-Aldrich

氯化钠, Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)

Sigma-Aldrich

氯化钠溶液, 5 M in H₂O, BioReagent, Molecular Biology, suitable for cell culture

Sigma-Aldrich

氯化钠, BioXtra, ≥99.5% (AT)

Sigma-Aldrich

氯化钠, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

Sigma-Aldrich

氯化钠溶液, 0.9% in water, BioXtra, suitable for cell culture

Sigma-Aldrich

氯化钠溶液, 5 M

SAFC

氯化钠溶液, 5 M

Sigma-Aldrich

亚甲蓝, certified by the BSC

Sigma-Aldrich

亚甲蓝溶液, concentrate according to Ehrlich, concentrated, aqueous solution

Sigma-Aldrich

氯化钠, BioUltra, Molecular Biology, ≥99.5% (AT)

Sigma-Aldrich

氯化钠, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

Sigma-Aldrich

氯化钠溶液, BioUltra, Molecular Biology, ~5 M in H₂O

Sigma-Aldrich

氯化钠, 99.999% trace metals basis

Supelco

氯化钠, Pharmaceutical Secondary Standard; Certified Reference Material

Sigma-Aldrich

N-苯基硫脲, ≥98%

Supelco

氯化钠, reference material for titrimetry, certified by BAM, >99.5%

Sigma-Aldrich

亚甲蓝溶液, 0.05 wt. % in H₂O

Sigma-Aldrich

氯化钠溶液, 0.85%

Sigma-Aldrich

氯化钠, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture

Sigma-Aldrich

氯化钠, tested according to Ph. Eur.

Sigma-Aldrich

亚甲蓝溶液, suitable for microscopy

Sigma-Aldrich

亚甲蓝溶液, suitable for microbiology

Sigma-Aldrich

氯化钠, tablet

Sigma-Aldrich

氯化钠-³⁵Cl, 99 atom % ³⁵Cl

Sigma-Aldrich

氯化钠, random crystals, 99.9% trace metals basis

Sigma-Aldrich

氯化钠, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

Sigma-Aldrich

氯化钠, Vetec^™, reagent grade, 99%