Human Keratinocytes Respond to Extracellular UTP by Induction of Hyaluronan Synthase 2 Expression and Increased Hyaluronan Synthesis.

Human Keratinocytes Respond to Extracellular UTP by Induction of Hyaluronan Synthase 2 Expression and Increased Hyaluronan Synthesis.

The Journal of biological chemistry (2017-02-12)

Tiina Jokela, Riikka Kärnä, Leena Rauhala, Genevieve Bart, Sanna Pasonen-Seppänen, Sanna Oikari, Markku I Tammi, Raija H Tammi

PMID28188289

摘要

The release of nucleotides into extracellular space is triggered by insults like wounding and ultraviolet radiation, resulting in stimulatory or inhibitory signals via plasma membrane nucleotide receptors. As similar insults are known to activate hyaluronan synthesis we explored the possibility that extracellular UTP or its breakdown products UDP and UMP act as mediators for hyaluronan synthase (

材料

产品编号

品牌

产品描述

D5921

Sigma-Aldrich

杜氏改良 Eagle 培养基-低葡萄糖, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

513000

Sigma-Aldrich

PD 98059, PD 98059, CAS 167869-21-8, is a cell-permeable, selective & reversible inhibitor of MAP Kinase Kinase (MEK). Inhibits MAP Kinase activation and subsequent phosphorylation of MAP Kinase substrates.

203290

Sigma-Aldrich

双辛基马来酰亚胺I, A highly selective, cell-permeable, and reversible protein kinase C (PKC) inhibitor (IC₅₀ = 10 nM) that is structurally similar to staurosporine.

422708

Sigma-Aldrich

KN-93, A cell-permeable, reversible and competitive inhibitor of rat brain CaM kinase II (K_i = 370 nM).

573125

Sigma-Aldrich

STAT3 Inhibitor IX, Cpd188, The STAT3 Inhibitor IX, Cpd188, also referenced under CAS 823828-18-8, controls the biological activity of STAT3.