GC Analysis of FAMEs in Atlantic Salmon on Omegawax
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CONDITIONS
column
Omegawax, 30 m x 0.25 mm I.D., 0.25μm (24136)
oven
170 °C, 1 °C/min to 225°C
inj. temp.
250 °C
detector
FID, 260 °C
carrier gas
helium, 1.2 mL/min
injection
1 μL, 100:1 split
liner
4 mm ID FocusLiner with taper
sample
1. Fish Oil from capsule, prepared according to AOAC Method 991.39 2. Atlantic, farm-raised salmon, prepared according to AOCS Official Method Ce 1k-09
说明
分析说明
Samples of farm-raised Atlantic salmon were prepared and analyzed for fatty acids. The salmon was extracted using an acid digestion /alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09 (2). The resulting extracts were then analyzed by GC/FID on an Omegawax 250 capillary column as described in AOCS Official Method Ce 1i-07.
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