GC Analysis of Short and Long Chain Free Fatty Acids on Nukol

column

Nukol, 15 m x 0.53 mm I.D., 0.50 μm (25326)

oven

100 °C, 10 °C/min to 220 °C

detector

FID

carrier gas

helium, 30 mL/min

injection

0.5 μL, direct injection

sample

16 analytes, at various concentrations from 50 to 800 μg/mL