SPE (Solid Phase Extraction)
1 g of commercially purchased cookies was ground and subjected to acid digestion and alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09
Discovery Ag-Ion SPE tubes, 750 mg/6 mL (54225-U)
4 mL of acetone; allow solvent to gravity drip completely through tube; discard eluant; 4 mL of hexane; allow solvent to gravity drip completely through tube; discard eluant
1 mL of extract; discard any eluant that drips through tube
(Fraction 1) 6 mL of hexane:acetone (96:4); collect eluant in a fresh container with slight vacuum; ((Fraction 2) 4 mL of hexane: acetone (90:10); collect eluant in a fresh container with slight vacuum;(Fraction 3) 4 mL of 100% acetone; collect eluant in a fresh container with slight vacuum)
evaporate each fraction at room temperature using nitrogen; reconstitute each fraction to 1 mL of hexane
SP-2560, 100 m x 0.25 mm I.D., 0.20 μm (24056)
180 °C
250 °C
FID, 250 °C
hydrogen, 1 mL/min
1 μL, 10:1 split
4 mm I.D., split type, single taper wool packed FocusLiner™ design
A sample of commercially purchased cookies were prepared using an acid digestions /alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09 (1). The resulting extracts were then fractionated using Ag-ion SPE and analyzed by GC/FID on the 100 m SP-2560 capillary column.
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