SPE (Solid Phase Extraction)
LpDNPH Cartridge (21024-U)
5 μg each carbonyl-DNPH on LpDNPH cartridge. Cartridge eluted with 5.0 mL acetonitrile
SUPELCOSIL LC-18, 25 cm × 4.6 mm I.D., 5 μm particles (58298)
[A]:acetonitrile:tetrahydrofuran:water, 30:10:60; [B]: acetonitrile:water, 60:40
gradient 0% B for 1 min, linear gradient to 100% B over 10 min
1.5 mL/min
VIS, 360 nm
25 μL of extract
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