HPLC Analysis of Acidic and Neutral Drug Panel on Ascentis^® Express C18

column

Ascentis Express C18, 10 cm × 4.6 mm I.D., 2.7 μm particles (53827-U)

mobile phase

[A] water with 0.1% phosphoric acid, [B] acetonitrile with 0.1% phosphoric acid

flow rate

1.76 mL/min

column temp.

ambient

detector

UV, 215 nm

injection

100 μL