HPLC Analysis of Acidic Drugs on Chromolith^® Performance 100- 4 mm (analyte set 1

column

Chromolith^® Performance 100 x 4 mm (1.52028.0001)

mobile phase

[A] acetonitrile; [B] ammoniumacetate 20mM pH=6,8; (28:70, A:B)

flow rate

2 mL/min (u=2mm/s)

pressure

14001 psi (97 bar)

column temp.

ambient

detector

230 nm (cell: 1.4 μL)

injection

1 μL

sample

Indoprofen, Naprofen, Ethacrynic acid, Ibuprofen, Indomethacine