HPLC Analysis of Acrylic Acid Derivatives on Discovery^® Zr-Carbon

column

Discovery Zr-Carbon, 7.5 cm × 2.1 mm I.D., 3 μm particles (65726-U)

mobile phase

[A] 30 mM phosphoric acid, pH 1.9; [B] acetonitrile

flow rate

0.42 mL/min

column temp.

50 °C

detector

UV, 210 nm (200 nm will enhance sensitivity ~2x)

injection

1 μL

sample

acrylate @ 0.05 g/L, methacrylate @ 0.05 g/L, sulfopropylacrylate @ 0.02 g/L, sulfopropylmethylacrylate @ 0.02 g/L in 30 mM phosphoric acid, pH 1.9