HPLC Analysis of Albuterol Tablets – Assay on Chromolith^® HighResolution RP-18 endcapped 150 x 4.6mm

sample preparation

120 μg/mL of Albuterol Sulfate RS prepared as follows. Transfer USP Albuterol Sulfate RS to a suitable volumetric flask, and add a volumne of Solution A corresponding to 60% of the flask volume. Sonicate for 5 minutes, and dilute with Methanol to volume.

column

Chromolith^® HighResolution RP-18 endcapped, 150 x 4.6 mm (1.52023.0001)

mobile phase

[A] 10mL/L of glacial acetic acid in water; [B] 1.13g of sodium 1-Hexanesulfate in 1200mL water, add 12mL of glacial acetic acid; (40:60,:A:B)

flow rate

1.5 mL/min

pressure

2001 psi (138 bar)

column temp.

ambient

detector

276 nm cell: 11 μL

injection

25 μL

sample

Albuterol RS 30 μg/mL in diluent (30 μg/mL of Albuterol Sulfate RS in Diluent from Standard stock solution)