HPLC Analysis of Amino Acids on Ascentis^® Si (Silica), HILIC Mode

column

Ascentis Si, 15 cm × 2.1 mm I.D., 5 μm particles (581509-U)

mobile phase

[A] 100 mM ammonium formate (pH 3.0, with formic acid), [B] water, [C] acetonitrile

flow rate

0.3 mL/min

column temp.

35 °C

detector

ESI (+), full scan

injection

2 μL

sample

in 10:90, (50 mM ammonium formate/formic acid, pH 3.0):acetonitrile