HPLC Analysis of Anions and Cations on SeQuant^® ZIC-HILIC

column

Sequant ZIC-HILIC, PEEK, 150 x 2.1 mm, 3.5 μm, 200 Å (1.50448.0001)

mobile phase

[A] acetonitrile; [B] 20 mM ammonium formate, pH 3

gradient

80% A held for 3 min; to 20% A in 7 min; held at 20% A for 3 min; to 80% A in 2 min; held at 80% A for 10 min

flow rate

0.3 mL/min

column temp.

40 °C

detector

ELSD (SEDEX 85LT, 40°C, 3.5 bar)

injection

2 μL