HPLC Analysis of Antibiotics (Fluoroquinolones from Tablets) on Discovery^® HS C18

column

Discovery HS C18, 15 cm × 4.6 mm I.D., 5 μm particles (568522-U)

mobile phase

[A] 25 mM potassium phosphate (pH 3.0); [B] acetonitrile

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 220 nm

injection

10 μL