Ascentis Express HILlC, 5 cm x 3.0 mm I.D., 2.7 μm particles (53967-U)
[A] 100 mM ammonium formate, pH 3.0 with formic acid; [B] acetonitrile; (10:90, A:B)
0.6 mL/min
25 °C
ELSD, 55 °C, 3.5 bar nitrogen
2 μL
1000 μg/mL in 10:90, water: acetonitrile
This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of caffeine and taurine. Elution is by aqueous normal phase (ANP) as an increase in organic will increase retention.
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany