HPLC Analysis of Capsacinoids in a Capsicum annuum L. extract.on Ascentis® Express C18
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CONDITIONS
column
Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm (53829-U)
mobile phase
[A] 0.075% acetic acid, pH 3; [B] 0.075% acetic acid in acetonitrile; (40:60; A:B)
flow rate
1 mL/min
detector
UV, 280 nm
injection
5 μL
说明
分析说明
Two grams of Capsicum annuum L. pericarp, placenta, and seeds were separately homogenized and extracted with 50 mL of water/methanol (25/75, v/v) for 20 minutes at 100 °C. The extracts were filtered and evaporated at 45 °C then resuspended in 1 mL of water/methanol (1/1, v/v).
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany