HPLC Analysis of Catechins on Ascentis^® RP-Amide

column

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

[A] 10 mM ammonium formate (pH 3.0 with concentrated formic acid); [B] acetonitrile

flow rate

1 mL/min

column temp.

35 °C

detector

MS, ESI (-) in selected ion recording (SIR) mode

injection

5 μL

sample

10 μg/mL each in water:acetonitrile 1:1