Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)
[A] 20 mM phosphoric acid (pH 2.0 unadjusted): [B] acetonitrile; (75:25, A:B)
1.5 mL/min
30 °C
UV, 270 nm
25 μL
as indicated in 20 mM phosphoric acid (pH 2.0)
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany