HPLC Analysis of Cefixime and Ofloxacin in Tablets on Chromolith® High Resolution RP-18e
CONDITIONS
sample preparation
Crush 20 tablets. Weigh equivalent to one tablet quantity & transfer to 100 mL volumetric flask. Add 50 ml diluent & sonicate for 20 mins. Dilute up to the mark the same. Further dilute with 2.5 mL of this solution to 100 mL. Filter through 0.2 μm filter
mobile phase
[A] 3.4 g of potassium dihydrogen phosphate in 1000 ml MilliQ water. Mix & sonicate it. Filter it with 0.2 μm filter; [B] acetonitrile; 87:13, A:B)
flow rate
1.0 mL/min
pressure
764 psi (53 bar)
column temp.
25 °C
detector
290 nm cell: 10 μL
injection
5 μL
sample
Weigh 5.0 mg of Cefixime & 5.0 mg of Ofloxacin refernce standard in 100 mL volumetric flask. Add about 50 mL diluent & sonicate till it dissolve. Dilute it upto the mark with the same. (Diluent: Dissolve 7.1 g of di-sodium hydrogen phosphate in 500 ml MilliQ water. Adjust pH at 7.0 with ortho-phosphoric acid. Mix & sonicate it. Filter it with 0.2 μm filter.)
column
Chromolith® High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)
说明
分析说明
Sample: 50 ppm (0.050 mg/mL) of each cefixime and ofloxacin in diluent.
Diluent: 7.1 g of disodium hydrogen phosphate in 500 mL Milli-Q water. pH 7.0 with orthophosphoric acid.
Method developed in house for tablet analysis.
法律信息
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany