HPLC Analysis of Chlorzoxazone on Discovery^® HS-PEG

column

Discovery HS PEG, 15 cm × 4.6 mm I.D., 5 μm particles (567416-U)

mobile phase

[A] 20 mM acetic acid in water (pH 4.5 with ammonium hydroxide):[B] acetonitrile; (70:30, A:B)

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 285 nm

injection

10 μL