HPLC Analysis of Cresols and Phenol on Astec^® CYCLOBOND^™ I 2000

column

CYCLOBOND I 2000, 25 cm x 4.6 mm I.D., 5 μm particles (20024AST)

mobile phase

[A] water; [B] methanol; (90:10, A:B)

flow rate

1 mL/min

column temp.

22 °C

detector

UV, 254 nm

injection

1 μL

sample

0.5 mg/mL in methanol

peak 1: o-cresol (10.70 min)

peak 2: phenol (11.70 min)

peak 3: m-cresol (14.20 min)

peak 4: p-cresol (20.10 min)