HPLC Analysis of Diuretic Drugs on Ascentis^® RP-Amide

column

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

[A] 10 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid); [B] acetronitrile; (60:40, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 234 nm

injection

10 μL

sample

50 μg/mL each in mobile phase