HPLC Analysis of Flavones on Discovery^® HS PEG

column

Discovery HS PEG, 15 cm × 4.6 mm I.D., 5 μm particles (567416-U)

mobile phase

[A] 0.1% formic acid in water; [B] 0.1% formic acid in methanol; (45:55, A:B)

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 254 nm

injection

10 μL, each compound 50 μg/mL