HPLC Analysis of Glyocoalkaloids Solanine and Cacoline on Chromolith^® NH2

column

Chromolith NH2, 5 cm x 4.6 mm I.D. (1.52027.0001)

mobile phase

[A] acetonitrile; [B] phosphate buffer; pH 6 (80:20, A:B)

flow rate

2 mL/min

column temp.

23 °C

detector

UV, 208 nm

injection

8 μL

sample

solanine 0.2 mg/mL, cacoline 0.2 mg/mL, in mobile phase

pressure

174 psi (12 bar)