HPLC Analysis of Hippuric and Methylhippuric Acids in Urine on SUPELCOSIL^™ LC-18

材料

3 μm particle size, L × I.D. 7.5 cm × 4.6 mm

SUPELCOSIL LC-18, 7.5 cm × 4.6 mm I.D., 3 μm particles (58984)

[A] tetrahydrofuran:10 mM potassium phosphate, pH 3.0 [B] with phosphoric acid (3:97, A:B)

1.5 mL/min

UV, 260 nm

10 μL spiked urine (300 μg/mL HA, o-MHA; 150 μg/mL m-MHA, p-MHA)

Urinary excretion of hippuric acid and m-or p-methylhippuric acid in the urine is a test of exposure to toluene and m-or p-xylene vapors.

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC