HPLC Analysis of Huaijiao Wan on Purospher^® Star Phenyl (5μm) 150 x 4.6 mm

column

Purospher^® Star Phenyl (5μm) 150 x 4.6 mm (1.51919.0001)

mobile phase

[A] 2% glacial acetic acid solution; [B] mix methanol and acetonitrile 2:1 (v/v);

gradient

Time (min): 0, 23, 50, 51, 57; A (%): 89, 89, 70, 10, 10; B (%): 11, 11, 30, 90, 90.

flow rate

1.0 mL/min

pressure

1131 psi (78 bar)

column temp.

50 °C

detector

280 nm cell: 1 μL/10 mm

injection

10 μL

sample

Dissolve appropriate amount of Sophoricoside, Naringin & Baicalin in methanol solution to obtain a 40, 20, 48 μg/mL (ppm) solution.