HPLC Analysis of α-Hydroxy Aliphatic Acids on Discovery^® RP-Amide C16

column

Discovery RP-AmideC16, 15 cm × 4.6 mm I.D., 5 μm particles (505013)

mobile phase

25 mM potassium phosphate, pH 3.0

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 220 nm

injection

10 μL