HPLC Analysis of Hydroxymetoprolol, Metoprolol and Alprenolol on Discovery^® HS F5

column

Discovery HS F5, 5 cm × 2.1 mm I.D., 3 μm particles (567500-U)

mobile phase

5 mM ammonium acetate in 10:90 water:acetonitrile

flow rate

0.2 mL/min

column temp.

60 °C

detector

(+) ESI

injection

10 μL

sample

as indicated (in mobile phase)