HPLC Analysis of Insulin on Chromolith^® Performance 100-2mm

sample preparation

9mg Insulin are dissolved in 9ml 0,01M HCl and put into the vaccuumdryer at 50°C for 4 hours. Then it was stored 96h by 25°C.

column

Chromolith^® Performance 100 x 2 mm (1.52006.0001)

mobile phase

[A] acetonitrile; [B] Water + 0,1% TFA v/v; (30:70, A:B)

flow rate

95 μL/min

pressure

1581 psi (109 bar)

column temp.

ambient

detector

215 nm cell: 1.4 μL

injection

0.4 μL

sample

Insulin 1mg/mL in 10mM HCl