HPLC Analysis of Insulin Variants and Analogs on BIOshell™ A160 Peptide C18
CONDITIONS
column
BIOshell A160 Peptide C18, 15 cm x 2.1 mm, 2.7 μm particles (66905-U)
mobile phase
[A] 75:25 water (0.1% trifluoroacetic acid) : acetonitrile (0.1% trifluoroacetic acid); [B] 50:50 water (0.1% trifluoroacetic acid) : acetonitrile (0.1% trifluoroacetic acid); (67:33, A:B)
flow rate
0.5 mL/min
pressure
3600 psi (248 bar)
column temp.
75 °C
detector
UV, 215 nm
injection
0.5 μL
sample
mix of six insulin variants, 100 μg/mL in 0.1% trifluoroacetic acid
说明
分析说明
BIOshell A160 Peptide C18 is an ideal column for reversed-phase separations of small proteins to large peptide sequences. There is over 75% sequence similarity between all six of the insulin variants in the sample; therefore, separation of these variants is achieved through the subtle differences in the polarities of surface residues on the peptides.
法律信息
BIOshell is a trademark of Sigma-Aldrich Co. LLC