HPLC Analysis of Light Scattering Detection of BSA on Zenix® SEC-300
CONDITIONS
column
Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles, 300 Å (Z777033)
mobile phase
0.1M phosphate buffer
flow rate
1.0 mL/min
column temp.
25 °C
detector
Hitachi-Elite LaChromL-2130 pump equipped with amulti-angle light scattering detector (DAWN-HELIOS: Wyatt Technology),a refractive index detector (Hitachi L-2490) and a UV-Vis detector (Hitachi 2420)
injection
20 μL
sample
BSA (20mg/mL)
说明
分析说明
The chromatogram shows an overlay of the ultraviolet (UV), differential refractive index (RI) and multi angle light scattering data (MALS) for a sample of bovine serum albumin; showing the prsenece of dimer and trimer as well as the monomer. The chromatogram illustrates the power of light scattering used with Sepax′s Zenix® SEC in protein characterization. The light scattering data makes it possible to determine the molecular mass for the monomer, dimer and trimer.
其他说明
Acknowledgements and thanks to Matthew Thompson for this data.
Postdoctoral Researcher - Gianneschi Lab, Department of Chemistry & Biochemistry University of California, San Diego
法律信息
Zenix is a registered trademark of Sepax Technologies