HPLC Analysis of Magnesium Ascorbyl Phosphate in Topical Cream on Chromolith^® NH2 50 - 4.6mm

sample preparation

Creme: 1,0 gr sample in 20mL Tetrahydrofuran / Buffer pH 4,0 (30/70) was diluted and stirred until completely homogeneous. The solutions obtained were injected directly into the chromatographic system.

column

Chromolith^® NH2 50 x 4.6 mm (1.52027.0001)

mobile phase

A] acetonitrile; [B] na-buffer 10mM pH=4.0; (20:80, A:B)

flow rate

2 mL/min

pressure

421 psi (29 bar)

column temp.

23 °C

detector

255 nm cell: 11 μL

injection

1 μL

sample

Magnesiumascorbylphosphate 100μg/ml, Aniti aging creme 1g/20ml