HPLC Analysis of Magnesium Ascorbyl Phosphate in Topical Cream on Chromolith® NH2 50 - 4.6mm
CONDITIONS
sample preparation
Creme: 1,0 gr sample in 20mL Tetrahydrofuran / Buffer pH 4,0 (30/70) was diluted and stirred until completely homogeneous. The solutions obtained were injected directly into the chromatographic system.
column
Chromolith® NH2 50 x 4.6 mm (1.52027.0001)
mobile phase
A] acetonitrile; [B] na-buffer 10mM pH=4.0; (20:80, A:B)
flow rate
2 mL/min
pressure
421 psi (29 bar)
column temp.
23 °C
detector
255 nm cell: 11 μL
injection
1 μL
sample
Magnesiumascorbylphosphate 100μg/ml, Aniti aging creme 1g/20ml
说明
法律信息
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany