HPLC Analysis of Milk Proteins on HiTrap^® SP

column

HiTrap SP, 1 mL (54817)

mobile phase

[A] 50 mM sodium acetate, pH 6.0; [B] 50 mM sodium acetate/1.0 M sodium chloride, pH 6.0

flow rate

1 mL/min

detector

UV, 280 nm

injection

0.5 mL filtered casein-precipitated human milk (buffer exchanged to mobile phase A)