HPLC Analysis of N-Linked Glycans on BIOshell Glycan

column

BIOshell Glycan HPLC
Column (50993-U)

mobile phase

(A) Water with 100 mM ammonium formate, pH 4.5; (B) Acetonitrile

gradient

min, %B, Flow (mL/min): 0-74-0.4; 15-71-0.4; 25-55-0.4; 26-45-0.2; 30-45-0.2; 31-75-0.4; 35-75-0.4.

pressure

1130 psi (78 bar)

column temp.

50 °C

detector

Fluorescence (&#955ex = 260 nm, &#955em = 430 nm)

injection

3 μL

sample

in 70% mobile phase B