HPLC Analysis of NSAIDs on Ascentis^® RP-Amide

column

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

[A] 10 mM dibasic potassium phosphate (pH 3.0): [B] acetonitrile; (45:55, A:B)

flow rate

1.0 mL/min.

column temp.

35 °C

detector

UV, 230 nm

injection

5 μL

sample

as indicated in 10 mM dibasic potassium phosphate (pH 3.0)