HPLC Analysis of NSAIDs on Discovery^® Zr-Carbon C18

column

Discovery Zr-Carbon C18, 15 cm × 4.6 mm I.D., 3 μm particles (65706-U)

mobile phase

[A] 50 mM phosphoric acid (pH 1.75): [B] acetonitrile; (50:50, A:B)

flow rate

4 mL/min

column temp.

80 °C

detector

UV, 254 nm

injection

1 μL

sample

ketoprofen, ibuprofen, naproxen, each 1 mg/mL