HPLC Analysis of Organic Acids on Chromolith^® High Resolution RP-18e

column

Chromolith^® High Resolution RP-18 encapped, 100 x 4.6 mm (1.52022.0001)

gradient

100% B for 0.5 min; to 82% B, hold until 5 min; to 100% B in 1 min; held at 100% B for 2.5 min, to 55% B for 3.5 mins, hold until 16 mins. (Initial composition: 0-0.5min 100%B to 82%B, hold until 5min, 5-6min 82%B to 100%B, hold until 8.5min, 8.5-12min 100%B to 55%B, hold until 16 min)

flow rate

1.0 mL/min

column temp.

ambient

detector

220 nm cell: 11μL

injection

5 μL

sample

Malic acid (0,5mg/ml), Fumaric acid (0,15mg/ml), Glutaric acid (0,7mg/ml), 3,4-Dihydroxy-cinnamic acid (0,1mg/ml), 4-Hydroxy-cinnamic acid (0,1mg/ml), Sorbic acid (0,75mg/ml), Benzoic acid (0,2mg/ml), 2-Hydroxybenzoic acid (0,5mg/ml), Cinnamic acid (0,2mg/ml)