HPLC Analysis of Peptides on Discovery® BIO PolyMA-SCX (Ion Exchange)
CONDITIONS
column
Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm I.D., 5 μm particles (59601-U)
mobile phase
([A] 5% acetonitrile in 20 mM ammonium carbonate, pH 3.5 with phosphoric acid; [B] 5% acetonitrile in 20 mM ammonium carbonate, 480 mM ammonium phosphate, pH 3.5 with phosphoric acid)
gradient
0 to 100% B in 24 min (linear)
flow rate
0.2 mL/min
column temp.
35 °C
detector
UV, 210 nm
injection
10 μL
sample
RP Peptide Ionic Interactions Standard, p/n RPS-10020 (Alberta Peptide Institute)
说明
分析说明
Polymer particles are frequently used in bioseparations. Their advantages over inorganic particles, however, are often offset by generally lower efficiency. Discovery BIO PolyMA-SCX and PolyMA-WAX particles offer the benefits of polymeric particles, but have higher efficiency and better resolution than competitive particles. An example of the high efficiency of Discovery BIO PolyMA-SCX is shown in this Figure. The Discovery BIO PolyMA-SCX column gives efficient, well-resolved separation of a four-component peptide mixture.
法律信息
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany