HPLC Analysis of Phenytoin on Chromolith^® Performance 250 - 4.6 mm

sample preparation

Mobile phase

column

Chromolith^® Performance 250 x 4.6 mm (1.02129.0001)

mobile phase

[A] metanol; [B] water; (50:50, A:B)

flow rate

0.5 mL/min

pressure

406 psi 28 Bar

column temp.

ambient

detector

229 nm cell: 10 μL

injection

10 μL

sample

1 ppm of each Phenytoin & all related impurities